Protein Synthesis Using a Reconstituted Cell‐Free System
نویسندگان
چکیده
منابع مشابه
Integration of a reconstituted cell-free protein-synthesis system on a glass microchip.
Recently, a cell-free protein synthesis system reconstituted solely from essential elements of the Escherichia coli translation system, termed protein synthesis using recombinant elements (PURE), has been widely used in synthetic biology to analyze fundamental life systems. Here, the system was integrated on a glass microchip system to construct a simple protein synthesis system. GFP template D...
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15 صفحه اولProtein translocation into proteoliposomes reconstituted
| We have reproduced the process of protein transport across and of protein integration into the mammalian endoplasmic reticulum membrane by the use of proteoliposomes reconstituted from pure phospholipids and purified membrane proteins. The transport of some proteins requires only two membrane protein complexes: the signal recognition particle receptor, needed for targeting of a nascent chain ...
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ژورنال
عنوان ژورنال: Current Protocols in Molecular Biology
سال: 2014
ISSN: 1934-3639,1934-3647
DOI: 10.1002/0471142727.mb1631s108